Speaker describes an experiment in mice where a particular fatty acid is injected to test whether it can reproduce patient-like symptoms, focusing on fatigue. Because mice cannot report subjective symptoms like brain fog, the experiment uses a behavioral proxy: trained mice run on murine treadmills and after repeated injections the investigators measure running persistence (number of stops) as an indicator of fatigue.
Then the next question was that forget about the bacteria, forget about the biofilm. If we can inject this particular fatty acid in animals, and what animals here we have used mice, then can we recapitulate or, or simulate some type of symptoms that, that, you know, you see in the patient? Now, obviously, the mice cannot talk about whether they have brain fog or not, but one thing that they we can study is fatigue. And there are multiple ways to study animal fatigue. One of them, which is shown in this paper, and there is a very nice sweet video, is that you can train the animals. Mice are very clever. You can train them how to run on treadmills. There are murine treadmills. So they will run on the treadmills, okay? And so you have to practice them, make the practice. And now you inject them with the stern home over a period of time and then see are they running on the treadmill in the similar way or are they stopping more? So if they're stopping more, that's an indicative of that they are getting tired.
