Separating Stem Cells during the BMAC

So we're transferring the bone marrow that we collected through this filter here. And this filter had heparin ran through it also to help with clotting.

And we'll transfer it into our red top tube here.

And then we'll shoot a little bit more heparin just to clear the line here.

We weigh them to make sure they're within three grams of one another.

And we shut the lid for our first spin.

So the first spin is probably three and a half minutes.

Does it, does it start till it revs up to the proper RPMs and.

Then it has to wind down well, so probably three and a half minutes and maybe eight minutes. Probably all together seconds to 10 to 15 minutes for the whole process.

The top layer, the almost yellowish color, that's going to be your platelets there that we separated from your whole blood, which is at the bottom now. So we'll extract all that out and there's a little buffy coat that lives on top of the whole blood that we'll get as well. What's in the buffy coat there? It's leukocytes, platelets, anti inflammatory.

All in one.

So we'll transfer it to this one.

This is going to be the second spin. We want to focus primarily on the platelets and mesenchymal stem cells.