Meduwa project - WP-Lead Meeting and GREAT-ER/WIS Session - 19 June 2019

Researchers tested plant powders and algae for antimicrobial properties to clean water and reduce antibiotic use. Bacillus subtilis proved susceptible, but water bacteria like Pseudomonas aeruginosa resisted. They're now growing new plants and testing how well they bind bacteria and antibiotics.
Chapters
Herbal mixtures and algae to clean the water and increase, resist disease resistance and replace antibiotics. We have got a lot of powders of different plants. What we have checked for antimicrobial substances, not the substances for themselves, but for the influence on the bacteria. And here you see the list of the different compounds. What we have checked. And we checked it by this method. We have added the bacteria, spread them all over the plate and added a disc with these herbal extracts in the middle. And then we measured the diameter of this inhibition soon. So this means here, when they grow all over the plate, then I have no antimicrobial effect. Here I have an antimicrobial effect. And this would be a very huge effect. And this we have differentiated in different periods. So this means we have taken the diameter or we have measured the diameters of the inhibition zone and then we have checked if these compounds are bactericidal. So this means bacteria which are taken off the plate or in the middle of the plate. We have plated them, replated them on plates which have no antibiotics and nothing else, only the medium to check if these bacteria have been dyed or if they are bacteriostatic. This means we took them out of the middle and looked if they only are inhibited by this growth or by this compound and can grow again. And here you see the tables. So this means the red one is. The red one is. With less dense, we have some growth. The yellow one is the bacteriostatic. And you see a lot of bacteriostatic compounds, the numbers of the diameter. Yeah, yeah. And on the topic, you see the different bacteria, what we have checked and what you see, a lot of bacteria have different resistance patterns. Bacillus subtilis, for example, is, will be killed by less antibiotic concentrations. So you have here a lot of yellow marks. Pseudomonas eritreanosa, which is a typical bacterium, is living in water, is stable and no compound is working on these bacteria. Also Enterobacter faecium as well. So we have no effect on this. So you have a species specific effect on these bacteria with these single compounds. And this is the next list. What we also have checked is the dilution, the micro dilution. So this means we have the dilution of the extracts and then added the bacteria in a special concentration. And then we looked to which concentration or to which dilution are these bacteria being able to grow. You also see here the green and the yellow. And here you see the greens are that they are killed and the bacteria static is the yellow one. And you see here a lot of different compounds which are working bacteria which are bacteriostatic and less they are killing these bacteria. Here is the next list. Just a minor question I ask you later. Okay. We are in trouble of time. So ongoing is we have got now Cornelis is producing new plants. These are growing and we are looking now if these plants are able to bind bacteria to bind antibiotics which are which should bind. But we will analyze these and we also analyze the chemical compounds in this water to know what is the background of the water and what is happening with the plants. Yes, these are the steps what are going on because the plants are growing. Now we have the first water sample, Scott. Now we are checking the microbiology and the chemical analyses are running and then we are looking when the plants are grown, we add some bacteria and we check if these are reduced or not. SA.
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